Frequency of Metallo-Β-Lactamase Among Pseudomonas aeruginosa Isolated from patients In Intensive Care Units and Operating Rooms

Abdulrahaman, Wafaa M. A. and Mohamed, Eldaw B. S. and Saidi, Fatimah M. S. and Elhassan, Mogahid M. (2023) Frequency of Metallo-Β-Lactamase Among Pseudomonas aeruginosa Isolated from patients In Intensive Care Units and Operating Rooms. Egyptian Academic Journal of Biological Sciences, G. Microbiology, 15 (1). pp. 1-11. ISSN 2090-0880

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Abstract

Background: P.aeruginosa infection is one of the major health problems in the world. It’s an opportunistic human pathogen that causes serious problems. Usually resistant to several antibiotics, show a particular ability to spread in hospitals. In recent times, Metallo-β-lactamase resistance in this bacterium has imposed some difficulties in treating bacterial infections. This study was a qualitative study, aimed to detect Metallo-β-Lactamase in Carbapenems-resistant P. aeruginosa isolated from inpatients admitted to Intensive Care Unit and Operating Room in different hospitals in Jeddah city, KSA during the period from March 2019 to September 2022.Methods: In this study, a total of (234) were cultured and identified using API 12A/12E. All isolates were subjected to antimicrobial susceptibility testing using Kirby Bauer method, for selected imipenem and meropenem. Results: Out of 234 specimens 154 Pseudomonas aeruginosa were isolated and identified, with different ratios (males, 62.3: females, 37.7), which occurred highest in the adult age group. The highest frequency of isolate 152 (98.7%) was in the intensive care unit while the lowest frequency of isolate 2 (1.3%) was in the operating room. (9%), (5.8%), (10.38%) and (1.2%) were found resistant to imipenem, meropenem, ciprofloxacin and amikacin respectively.Conclusion: Carbapenems have great bactericidal activity against Ps. aeruginosa, while, this notorious pathogen acquisition resistance against these drugs and limited treatment options. Our isolated strains showed a low rate of resistance (9%) and (5.8%) against imipenem and meropenem respectively. Aminoglycoside is crucial for the treatment of various Ps. aeruginosa infections. However, our study showed that (1.2%) of Ps. aeruginosa strains were resistances to amikacin and Ciprofloxacin has been extensively used to treat wide a variety of Ps. aeruginosa infections. While Ps. aeruginosa rapidly acquired resistance to ciprofloxacin that creating a therapeutic challenge. In this study, 10.38% of Ps. aeruginosa was found resistant to Ciprofloxacin. We recommended further studies and a regular monitoring system for the early detection of MBL-producing organisms.

Item Type: Article
Subjects: Middle East Library > Biological Science
Depositing User: Unnamed user with email support@middle-eastlibrary.com
Date Deposited: 10 Jul 2023 05:40
Last Modified: 06 Sep 2024 09:03
URI: http://editor.openaccessbook.com/id/eprint/1307

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