Phylogenetic Position of Xenoturbella bocki and Hemichordates Balanoglossus carnosus and Saccoglossus kowalevskii Based on Amino Acid Composition or Nucleotide Content of Complete Mitochondrial Genomes

To clarify the phylogenetic position of Xenoturbella bocki and hemichordates (Balanoglossus carnosus and Saccoglossus kowalevskii), which occupy an ambiguous evolutionary position between vertebrates and invertebrates, we expanded a pre-existing baseline vertebrate data set by incorporating randomly selected invertebrates. Based on nucleotide content calculated from complete mitochondrial genomes, invertebrates were classified into two groups, high C/G and low C/G, with vertebrates placed within the high-C/G invertebrate group. X. bocki and hemichordates (B. carnosus and S. kowalevskii) also fell into the high-C/G invertebrate group. We also analyzed amino acid composition and nucleotide content of complete mitochondrial genomes or 16S rRNA genes using Ward’s clustering method and neighbor-joining. In the resulting phylogenetic trees, invertebrates are classified into high C/G and low C/G categories, and vertebrates are divided into terrestrial and aquatic groups. In addition, X. bocki and representative hemichordates B. carnosus and S. kowalevskii appear to be more closely related to vertebrates than to invertebrates.